ANTIGENIC ANALYSIS OF JAPANESE ENCEPHALITIS VIRUS BY CROSS NEUTRALIZATION TEST
نویسندگان
چکیده
منابع مشابه
Antigenic analysis of Japanese encephalitis virus by using monoclonal antibodies.
Hybridoma cells were produced by fusing P3X63Ag8.653 mouse myeloma cells with spleen cells from BALB/c mice immunized with Japanese encephalitis (JE) virus, Nakayama-RFVL strain. The resulting 26 clones produced hemagglutination inhibition antibodies against the homologous strain. The hemagglutination inhibition reactivity of each clone was tested against six flaviviruses: JE, Murray Valley enc...
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Persistence of Wild-Type Japanese Encephalitis Virus Strains Cross-Neutralization 5 Years After JE-CV Immunization
Background The live-attenuated Japanese encephalitis (JE) vaccine (JE-CV; IMOJEV) induces a protective response in children. A shift in circulating JE virus strains suggests that a genotype shift phenomenon may occur throughout Southeast Asia. We assessed the neutralization of wild-type (WT) JE virus isolates at distal time points after vaccination. Methods We analyzed serum samples from a su...
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Geographical expansion and re-emerging new genotypes of the Japanese encephalitis virus (JEV) require the development of novel therapeutic approaches. Here, we studied a non-conventional approach for antibody therapy and show that, upon exposure to heme, a fraction of natural human immunoglobulins acquires high-affinity reactivity with the antigenic domain-III of JEV E glycoprotein. These JEV-r...
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ژورنال
عنوان ژورنال: Uirusu
سال: 1968
ISSN: 0042-6857,1884-3433
DOI: 10.2222/jsv.18.6